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will eventually hamper its use Alternatively, the use of holins as part of bacteriophages looks like a very promising tool in the foreseeable future 62 Biotechnological Added Value of Holins Ever since their discovery, holins have been considered useful tools in the eld of biotechnology, in particular in areas such as food production and biological retention Food production often involves biological transformation processes that depend on the spillage to the media of critical enzymes through a controlled or an uncontrolled lysis event In this line, phage holin endolysin pairs cloned under inducible promoters in plasmids of Lactococcus lactic have shown to satisfactorily modulate the ef ciency of the lysis process with relevance in the dairy industry (de Ruyter et al 1997; Labrie et al 2004) This strategy can be also powerful for the effective elimination of food poisoning pathogens such as Listeria monocytogenes, Campylobacter jejuni, and Salmonella (Greer 2005).

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Dim b As New BarcodeLib . Barcode Dim TYPE As BarcodeLib .TYPE TYPE = BarcodeLib .TYPE.CODE39 Dim IMG As Image IMG = b.

for any lter P(z) M(L). Using the above properties, we derive the natural gradient learning algorithm for multichannel deconvolution. In order to implement the adaptive on-line learning, we formulate the standard cost function as

In fact, in August 2006, the Food and Drug Administration (FDA) approved the use of phages for the treatment of ready-to-eat meat, the process consisting of a mixture of six phages designed to be sprayed on the meat to eradicate strains of Listeria monocytogenes (Petty et al 2007) Similarly, the area of bioremediation centers its action on the exploitation of the metabolic power of different bacteria for environmental purposes (transforming a given contaminating compound, metabolizing a key substance, etc), such that after executing their action, the bacteria must die In this case, modifying such organisms with phages containing either a holin or the holin endolysin pair under the control of an inducible promoter sensitive to the substance of interest provides a suitable controlled system 63 Holins in Biomedicine In biomedicine, holins can be exploited as universal lethal cargos.

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It must be mentioned that the ectopic expression of the S holin in eukaryotic cells is cytotoxic, leading to cell death by a non-apoptotic mechanism (Agu et al 2006, 2007) S holin expression produces the disruption of the mitochondrial membrane potential and a massive vacuolization (Agu et al 2006, 2007) This nding anticipates the feasibility of holins as antitumoral agents in gene therapy Regarding the potential of holins to combat infectious diseases, their use is still limited to phage therapy as a holin-modi ed bacteriophage..

Organizing Layers for Clarity ............................................................................................445 Naming your layers ................................................................................................446 Grouping layers by content......................................................................................449

D = 2.500 X 10- 8

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